highly sensitive detection of hbv rna in liver tissue by in situ hybridization
Methods in molecular biology vol 1540 hepatitis b virus methods and protocols
Ngày tải lên :
24/12/2016, 22:30
... Nested PCR Thomas Tu and Allison R Jilbert 10 Highly Sensitive Detection of HBV RNA in Liver Tissue by In Situ Hybridization Diego Calabrese ... 3.7 Quantification of HBV- Specific RNAs Wash the cells with 200 μL 1× PBS once on day postinfection, add 200 μL Trizol reagent, and extract total RNA following the manual (RNA can also be extracted ... labeled probes to hybridization, or store at −20 °C (see Note 10) Place the crosslinked membrane in a hybridization tube to perform prehybridization in 5 mL PerfectHyb™ plus hybridization buffer...
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Báo cáo y học: "Management of HBV Infection in Liver Transplantation Patients"
Ngày tải lên :
02/11/2012, 11:17
... high HBV viral loads at the time of OLT, patients with active replication detected by insensitive molecular hybridization assays were considered as inferior candidates [9] Among patients receiving ... was only 5.2% (range 0-18%) after to years, [14,15,18,28,43,48,49,75,80,81] Importantly, using highly sensitive PCR, circulating HBV DNA was undetectable in most patients after OLT [7,28,82] The ... LAM-resistant mutations as a result of amino acid substitutions in the YMDD motif encoded by the HBV RNA- dependent DNA polymerase gene [54] The incidence of such mutations was 15-20% per year in the...
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Tài liệu Báo cáo khoa học: "Automatic Detection of Nonreferential It in Spoken Multi-Party Dialog" doc
Ngày tải lên :
22/02/2014, 02:20
... systems for written text include some means for the detection of nonreferential it However, evaluation figures for this task are not always given As the detection of nonreferential it is supposed to ... learned show that the features found to be most important for the detection of nonreferential it in written text (cf Section 2) are also highly relevant for performing that task for spoken language ... International Joint Workshop on Natural Language Processing in Biomedicine and its Applications, Geneva, Switzerland Cohen, W W (1995) Fast effective rule induction In Proc of the 12th International...
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Detection of Pseudomonas aeruginosa in sputum headspace through volatile organic compound analysis potx
Ngày tải lên :
05/03/2014, 21:20
... perform quantification for complex matrices, the use of internal standards or standard additions is recommended Using only a few internal standards, representing the main chemical classes and ... 40:715–720 14 Terpstra WJ, Schoone GJ, Ter SJ, van Nierop JC, Griffioen RW: In situ hybridization for the detection of haemophilus in sputum of patients with cystic fibrosis Scand J Infect Dis ... aeruginosa at study visit This suggests that gas chromatography – mass spectrometry may be more sensitive than bacterial culturing Previous studies have shown that several VOCs in sputa, breath...
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R"eCseoarcuh garthicl eofficer screening" improves detection of pulmonary tuberculosis in hospital in-patients pptx
Ngày tải lên :
06/03/2014, 04:20
... There were 42 patients (17 in the internal medicine department, in the surgical department, and 21 in the ICU) in stage I, and 12 patients (all in the internal medicine department) in stage II ... al reported a 35% TB detection rate among the 180 patients referred from a general outpatient department to a "chronic cough room" at a hospital in Blantyre, Malawi The high detection rate in this ... Chest Medicine, Department of Internal Medicine, Changhua Christian Hospital, 135 Nanshiao Road, Changhua, Taiwan, 2Division of Infectious Disease, Department of Internal Medicine, Changhua Christian...
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ag doped wo3-based powder sensor for the detection of no gas in air
Ngày tải lên :
19/03/2014, 16:47
... 89±92 [15] M Akiyama, J Tamaki, N Miura, N Yamazoe, Tungsten oxidebased semiconductor sensor highly sensitive to NO and NO2, Chem Lett (1991) 1611±1614 L Chen, S.C Tsang / Sensors and Actuators ... limited work reported in the literature on addressing the precise sensing mechanism for the NOx gases detection over WO3-based sensors and there is no work to reveal the roles of the promoter It is ... screening 1% doped WO3 sensors, pure tungsten trioxide sensor pellet was initially tested for the detection of NO in air in order to optimize acquisition parameters It was found that the resistance...
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Báo cáo " On the detection of gross errors in digital terrain model source data " pdf
Ngày tải lên :
22/03/2014, 12:20
... Tran Quoc Binh / VNU Journal of Science, Earth Sciences 23 (2007) 213‐219 References [1] A. Felicisimo, Parametric statistical method for error detection in digital elevation models, ISPRS Journal of ... work on correcting gross errors of DTM source data. ‐ After automated detection, a manual check Tran Quoc Binh / VNU Journal of Science, Earth Sciences 23 (2007) 213‐219 of marked points is still required for determining ... of error detection process. 2.2. Test procedure The workflow of the test is presented in Fig. 1. For the test, we have developed a simple software called DBD (DTM Blunder Detection) , ...
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The bleach method improves the detection of pulmonary tuberculosis in Laos ppt
Ngày tải lên :
29/03/2014, 03:20
... Nationale de l’Eau de Javel, Neuilly-sur-Seine, France 1128 The International Journal of Tuberculosis and Lung Disease References Arnadottir T H, Soukaseum H, Vangvichit P, Bounmala S, Vos E Prevalence ... differences in assessment of bacillary density All were resolved after rereading The 1126 The International Journal of Tuberculosis and Lung Disease Table Origin of sputum samples Patients (N = 612) Sputum ... Cunningham J, Perkins M D The bleach microscopy method and case detection for tuberculosis control Int J Tuberc Lung Dis 2006; 10: 256–258 International Union Against Tuberculosis and Lung Disease Sputum...
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Báo cáo hóa học: " Detection of epithelial apoptosis in pelvic ileal pouches for ulcerative colitis and familial adenomatous polyposis" pot
Ngày tải lên :
18/06/2014, 16:20
... g at 4°C) The protein concentrations of the supernatants were determined by the Bradford dye binding method [20] Aliquots of the resulting supernatants containing 100 μg total proteins were separated ... doi:10.1186/1479-5876-8-11 Cite this article as: Leal et al.: Detection of epithelial apoptosis in pelvic ileal pouches for ulcerative colitis and familial adenomatous polyposis Journal of Translational Medicine 2010 ... histological analysis to determine the expression of pro-apoptotic and anti-apoptotic proteins, and detection of apoptosis by Annexin V fluorescence microscopy in ileal pouch biopsies Methods Mucosal...
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Báo cáo khoa học: "Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR" pot
Ngày tải lên :
07/08/2014, 14:22
... [8, 18, 20] Recently, PCR/ Southern hybridization, and nested PCR assays for the detection of L intracellularis-specific DNA have been reported to be more sensitive than other conventional methods ... scraped from the ileum and homogenized using a tissue grinder The homogenate was centrifuged at 750 g for 10 at room temperature, and the supernatant was filtered sequentially through 5-µm, ... observation These results indicated that the one step amplification by PCR reaction was more sensitive for the detection of L intracellularis than the conventional method One fecal specimen was negative...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
Ngày tải lên :
07/08/2014, 17:22
... Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated virusa ... colorization, methyl green counterstain, Bar = 50 µm formula of hybridization solution was described previously [3] For detection of hybridization, sections were incubated with anti-digoxigenin ... proteinase K in situ hybridization for CHV In this study, several ISH protocols, which were consisted of the changes of enzyme-concentrations, the time of hybridization and hybridization probes,...
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Báo cáo khoa học: "Use of soft X-radiography for early non-destructive detection of floral differentiation in Douglas fir buds" pptx
Ngày tải lên :
08/08/2014, 19:21
... Sahlen, 1981; Chavagnat, 1984, 1985) Radiographs provide morphological information about organs or tissues which would otherwise be masked by others, while preserving plant specimens intact With ... and (ii) to have a more accurate definition of the state or the development stage of organs and tissues for sampling, especially for sparse material Studies on conifer bud initiation/differentiation ... Girdles were double, overlapping, half circumferential bands (5 mm wide) Root flooding was alternated (2.5 d in water with 2.5 d out of water over 15 d) Trees and were treated with hormonal injection...
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Báo cáo y học: "Conventional radiography requires a MRI-estimated bone volume loss of 20% to 30% to allow certain detection of bone erosions in rheumatoid arthritis metacarpophalangeal joints" ppsx
Ngày tải lên :
09/08/2014, 07:20
... reported recently that low field dedicated MRI, as used in the present study, is highly sensitive and specific for detection as well as grading of bone erosions when compared to standard high field ... conventional radiography for detection of MRI erosions of different sizes was calculated Figure resonance imaging erosions The sensitivity of conventional radiography for detection of magnetic resonance ... tested against computed tomography, which can be considered a standard reference for detection of loss of calcified tissue, the available data suggest a high agreement between MRI and computed tomography...
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Báo cáo khoa học: "Detection of somatostatin receptors in human osteosarcoma" pot
Ngày tải lên :
09/08/2014, 07:21
... World Journal of Surgical Oncology 2008, 6:99 http://www.wjso.com/content/6/1/99 In this study we aim to ... metastases) NED GH receptor status + + + + Page of (page number not for citation purposes) World Journal of Surgical Oncology 2008, 6:99 http://www.wjso.com/content/6/1/99 Table 2: Disease free and ... Hormone 19/16–24 years 28,32/16–49 years Page of (page number not for citation purposes) World Journal of Surgical Oncology 2008, 6:99 http://www.wjso.com/content/6/1/99 Table 4: Disease free and...
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Báo cáo y học: "Detection of bone erosions in rheumatoid arthritis wrist joints with magnetic resonance imaging, computed tomography and radiography" ppt
Ngày tải lên :
09/08/2014, 10:23
... three-dimensional visualisation of calcified tissue CT seems to be even more sensitive than MRI for detection of bone erosions, and can be considered a standard reference for detection of bone erosions in RA ... modalities have emerged as methods for more sensitive detection of early bone erosions [9-12] Magnetic resonance imaging (MRI) has been demonstrated to be more sensitive than radiography in detecting ... with MRI and radiography, indicate that CT is a very sensitive method for detecting bone erosions in RA wrist bones, and possibly even more sensitive than MRI CT may therefore be of value for...
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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
Ngày tải lên :
09/08/2014, 14:22
... preceding but asymptomatic infection [7,8] PCR using universal 16S rRNA primers is a highly sensitive tool allowing detection of unknown, that is unsuspected, pathogens relating to all eubacterial ... bacterial rRNA Arthritis Res Ther 2003, 5:R1-R8 Kempsell KE, Cox CJ, Hurle M, Wong A, Wilkie S, Zanders ED, Gaston JS, Crowe JS: Reverse transcriptase-PCR analysis of bacterial rRNA for detection ... and/or RNA [4-6] There is also evidence that undifferentiated arthritis is a form of ReA ('forme fruste') possibly due to a preceding but asymptomatic infection [7,8] PCR using universal 16S rRNA...
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báo cáo khoa học: "Electrophoretic detection of interspecific hybrids in Parnassius (Lepidoptera Papilionidae)" docx
Ngày tải lên :
09/08/2014, 22:22
... wing pattern One example of hybridization between butterfly species concerns Parnassius apollo L and P phœbus Fabr., two taxa that are generally ... difficulties have been met with in hybridization studies carried out in other zoological groups (N & SMITH, 1979 ; LAMB EFF & AvtsE, 1987) the Such natural hybridization events are of considerable ... has been roughly estimated to vary between and 10 % of the total Parnassius population in different years In the Bor6on region hybridization has been investigated for fewer years, but hybrids have...
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Báo cáo y học: "Effective detection of rare variants in pooled DNA samples using Cross-pool tailcurve analysis" ppsx
Ngày tải lên :
09/08/2014, 23:20
... variant detection in pooled samples of both cohorts as compared to publicly available algorithms Keywords Next-generation Sequencing, Sample-Pooling, Multiplexed Sequencing, Rare Variant Detection, ... lane of a GAII Illumina Sequencer We utilized an alternative base-calling algorithm, SRFIM [7], and an automated filtering program, SERVIC4E, (Sensitive Rare Variant Identification by Cross-pool ... Identification by Cross-pool Cluster, Continuity, and tailCurve Evaluation), designed for sensitive and reliable detection of rare variants in pooled samples We validated this strategy using Illumina...
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báo cáo khoa học: "mRNA detection of individual cells with the single cell nanoprobe method compared with in situ hybridization" doc
Ngày tải lên :
11/08/2014, 00:22
... nanoprobe method 0–0.1 β-actin mRNA detection probability Average number of detected β-actin mRNA Hybridization and detection by alkaline phosphatase reaction Hybridization solution (60% formamide ... of each figure are the β-actin mRNA quantity detected by the SCN method Scale bar is µm When the average of IISH was over 0.25, the probability of β-actin mRNA detection was 100% However, the ... indicate β-actin mRNA quantities detected by the SCN method, and dark intracellular staining indicates distribution of β-actin mRNA detected by ISH (d) Negative control using sense RNA probe Scale...
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